Nikon TIRF switch on

Required elements

4: Controller (this operates the microscope)

5: Internal cam (to inspect the objective back aperture to guarantee TIRF, software always requires this on)

Optional elements

1: Lasers (if you want to do TIRF or use lasers for widefield)

2: Warming box (if you want to have the box-light available or for live-cell incubation)

3: CO2 and humidity (for live-cell incubation)