Nikon TIRF switch on

Required elements

4) Controller (this operates the microscope)

5) Internal camera (Fi3, to inspect the objective back aperture to guarantee TIRF, software always requires this on)

6) Main camera (Prime BSI, to actually observe the sample through the objective lens)

7) Computer (for observation and imaging by camera, otherwise you can only use the eyepieces)

Optional elements

1) Lasers (if you want to actually do TIRF, or use the specific laser wavelengths for widefield epifluorescence)

2) Warming box (if you want to have the incubator box light available, and for if you want to do live-cell incubation)

3) Humidity + CO2 (for live-cell incubation)

-> When in doubt: Switch them all on