Nikon TIRF switch on: Difference between revisions

Revision as of 09:11, 10 June 2026

4) Controller (this operates the microscope)

5) Internal camera (Fi3, to inspect the objective back aperture to guarantee TIRF, software always requires this on)

6) Main camera (Prime BSI, to actually observe the sample through the objective lens)

1) Lasers (if you want to actually do TIRF, or use the specific laser wavelengths for widefield epifluorescence)

2) Warming box (if you want to have the incubator box light available, and for if you want to do live-cell incubation)

3) Humidity + CO2 (for live-cell incubation)

7) Computer (for observation and imaging by camera, otherwise you can only use the eyepieces)

-> When in doubt: Switch them all on

@@ Line 1: / Line 1: @@
 [[File:TIRF switch on.png|880x880px]]
+=== '''Required elements''' ===
+) '''Controller''' (this operates the microscope)
-Required elements
+) '''Internal camera''' (Fi3, to inspect the objective back aperture to guarantee TIRF, software always requires this on)
-: Controller (this operates the microscope)
+) '''Main camera''' (Prime BSI, to actually observe the sample through the objective lens)
-: Internal cam (to inspect the objective back aperture to guarantee TIRF, software always requires this on)
+=== '''Optional elements''' ===
+) '''Lasers''' (if you want to actually do TIRF, or use the specific laser wavelengths for widefield epifluorescence)
+) '''Warming box''' (if you want to have the incubator box light available, and for if you want to do live-cell incubation)
+) '''Humidity + CO2''' (for live-cell incubation)
-Optional elements
+) '''Computer''' (for observation and imaging by camera, otherwise you can only use the eyepieces)
-: Lasers (if you want to do TIRF or use lasers for widefield)
-: Warming box (if you want to have the box-light available or for live-cell incubation)
+'''-> When in doubt:''' Switch them all on
-: CO2 and humidity (for live-cell incubation)