Nikon TIRF widefield: Difference between revisions
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== Technical specifications == | == Technical specifications == | ||
'''Light sources and filters:''' | |||
'''Epifluorescence mode''' with LED light sources (''non-TIRF)'': Excitations: 385, 475, 550, 621 nm. Emissions: Band pass emission filters for blue (ca. 450), green (ca. 510), red (ca. 600) and far-red (ca. 670). | |||
'''TIRF-mode''' with lasers: Excitations: 488 nm, 561 nm, 640 nm. Emission filters: None | |||
Latest revision as of 15:17, 24 November 2025
Manuals and user guides
Current status
The current status of the microscope can be found here and on the booking website.
Biosafety and access
The TIRF is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.
Technical specifications
Light sources and filters:
Epifluorescence mode with LED light sources (non-TIRF): Excitations: 385, 475, 550, 621 nm. Emissions: Band pass emission filters for blue (ca. 450), green (ca. 510), red (ca. 600) and far-red (ca. 670).
TIRF-mode with lasers: Excitations: 488 nm, 561 nm, 640 nm. Emission filters: None