Leica SP8 Liachroic: Difference between revisions
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== Manuals and user guides == | |||
[[Leica SP8 Liachroic intro|Introduction protocol]] | |||
== Current status == | |||
The current status of the microscope can be found [[Current microscope status#Light microscopes|here]] and on the [https://bookings.science.ru.nl/category/general-instrumentation-gi/gi-light-microscopy-image-analysis booking website]. | |||
== Biosafety and access == | |||
The SP8-Lia is located in an AP-1 room. See the [[General Instrumentation Department#Biosafety|Biosafety]] section on rules regarding working in this room. | |||
== Technical specifications == | |||
* Inverted DMi8 CS motorized microscope | |||
* 2000 FOV scanner SP8 | |||
* Light source EL 6000 | |||
===Filter Cubes=== | |||
* DAPI LP (ex: 380-400, em: 425-infinite nm) | |||
* GFP (ex: 450-490, em: 500-550) | |||
* Y3 (ex: 533-558, em: 570-640) | |||
* Y5 (ex: 590-650, em: 662-738) | |||
===Objectives=== | |||
* Obj. HC PL APO 10x/0.40 CS2 (FWD = 2.56 mm) | |||
* Obj. HC PL FLUOTAR L 20x/0.40 (7.5-6.2 mm) | |||
* Obj. HC PL APO 20x/0.75 CS2 (0.66 mm) | |||
* Obj. HC PL FLUOTAR L 40x/0.60 (3.3-1.9 mm) | |||
* Obj. HC PL APO 40x/1.30 Oil CS2 (0.24 mm) | |||
* Obj. HC PL APO 63x/1.40 OIL CS2 (0.14 mm) | |||
===Detectors=== | |||
* 1 x PMT Detector | |||
* 2 x HyD SP GaAsP Detector (sensitive) | |||
* Brightfield detector for DMI | |||
===Lasers=== | |||
* Laser 405 nm DMOD Compact | |||
* Laser Blue 488 nm | |||
* Laser Green 552 nm | |||
* Laser Red 638 nm | |||
===Camera=== | |||
* Leica DFC7000 GT monochrome camera | |||
===Software=== | |||
* LAS X SP8 Control Software with Navigator Module | |||
Latest revision as of 11:19, 29 September 2025
Manuals and user guides
Current status
The current status of the microscope can be found here and on the booking website.
Biosafety and access
The SP8-Lia is located in an AP-1 room. See the Biosafety section on rules regarding working in this room.
Technical specifications
- Inverted DMi8 CS motorized microscope
- 2000 FOV scanner SP8
- Light source EL 6000
Filter Cubes
- DAPI LP (ex: 380-400, em: 425-infinite nm)
- GFP (ex: 450-490, em: 500-550)
- Y3 (ex: 533-558, em: 570-640)
- Y5 (ex: 590-650, em: 662-738)
Objectives
- Obj. HC PL APO 10x/0.40 CS2 (FWD = 2.56 mm)
- Obj. HC PL FLUOTAR L 20x/0.40 (7.5-6.2 mm)
- Obj. HC PL APO 20x/0.75 CS2 (0.66 mm)
- Obj. HC PL FLUOTAR L 40x/0.60 (3.3-1.9 mm)
- Obj. HC PL APO 40x/1.30 Oil CS2 (0.24 mm)
- Obj. HC PL APO 63x/1.40 OIL CS2 (0.14 mm)
Detectors
- 1 x PMT Detector
- 2 x HyD SP GaAsP Detector (sensitive)
- Brightfield detector for DMI
Lasers
- Laser 405 nm DMOD Compact
- Laser Blue 488 nm
- Laser Green 552 nm
- Laser Red 638 nm
Camera
- Leica DFC7000 GT monochrome camera
Software
- LAS X SP8 Control Software with Navigator Module