Leica SP8 Liachroic

Manuals and user guides

Introduction protocol

Current status

The current status of the microscope can be found here and on the booking website.

Biosafety and access

The SP8-Lia is located in an AP-1 room. See the Biosafety section on rules regarding working in this room.

Technical specifications

• Inverted DMi8 CS motorized microscope
• 2000 FOV scanner SP8
• Light source EL 6000

Filter Cubes

• DAPI LP (ex: 380-400, em: 425-infinite nm)
• GFP (ex: 450-490, em: 500-550)
• Y3 (ex: 533-558, em: 570-640)
• Y5 (ex: 590-650, em: 662-738)

Objectives

• Obj. HC PL APO 10x/0.40 CS2 (FWD = 2.56 mm)
• Obj. HC PL FLUOTAR L 20x/0.40 (7.5-6.2 mm)
• Obj. HC PL APO 20x/0.75 CS2 (0.66 mm)
• Obj. HC PL FLUOTAR L 40x/0.60 (3.3-1.9 mm)
• Obj. HC PL APO 40x/1.30 Oil CS2 (0.24 mm)
• Obj. HC PL APO 63x/1.40 OIL CS2 (0.14 mm)

Detectors

• 1 x PMT Detector
• 2 x HyD SP GaAsP Detector (sensitive)
• Brightfield detector for DMI

Lasers

• Laser 405 nm DMOD Compact
• Laser Blue 488 nm
• Laser Green 552 nm
• Laser Red 638 nm

Camera

• Leica DFC7000 GT monochrome camera

Software

• LAS X SP8 Control Software with Navigator Module