Leica SP8 Liachroic: Difference between revisions
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* 2000 FOV scanner SP8 | * 2000 FOV scanner SP8 | ||
* Light source EL 6000 | * Light source EL 6000 | ||
===Filter Cubes=== | |||
* DAPI LP (ex: 380-400, em: 425-infinite nm) | * DAPI LP (ex: 380-400, em: 425-infinite nm) | ||
* GFP (ex: 450-490, em: 500-550) | * GFP (ex: 450-490, em: 500-550) | ||
* Y3 (ex: 533-558, em: 570-640) | * Y3 (ex: 533-558, em: 570-640) | ||
* Y5 (ex: 590-650, em: 662-738) | * Y5 (ex: 590-650, em: 662-738) | ||
===Objectives=== | |||
* Obj. HC PL APO 10x/0.40 CS2 (FWD = 2.56 mm) | * Obj. HC PL APO 10x/0.40 CS2 (FWD = 2.56 mm) | ||
* Obj. HC PL FLUOTAR L 20x/0.40 (7.5-6.2 mm) | * Obj. HC PL FLUOTAR L 20x/0.40 (7.5-6.2 mm) | ||
| Line 26: | Line 27: | ||
* Obj. HC PL APO 40x/1.30 Oil CS2 (0.24 mm) | * Obj. HC PL APO 40x/1.30 Oil CS2 (0.24 mm) | ||
* Obj. HC PL APO 63x/1.40 OIL CS2 (0.14 mm) | * Obj. HC PL APO 63x/1.40 OIL CS2 (0.14 mm) | ||
===Detectors=== | |||
* 1 x PMT Detector | * 1 x PMT Detector | ||
* 2 x HyD SP GaAsP Detector (sensitive) | * 2 x HyD SP GaAsP Detector (sensitive) | ||
* Brightfield detector for DMI | * Brightfield detector for DMI | ||
===Lasers=== | |||
* Laser 405 nm DMOD Compact | * Laser 405 nm DMOD Compact | ||
* Laser Blue 488 nm | * Laser Blue 488 nm | ||
* Laser Green 552 nm | * Laser Green 552 nm | ||
* Laser Red 638 nm | * Laser Red 638 nm | ||
===Camera=== | |||
* Leica DFC7000 GT monochrome camera | * Leica DFC7000 GT monochrome camera | ||
===Software=== | |||
* LAS X SP8 Control Software with Navigator Module | * LAS X SP8 Control Software with Navigator Module | ||
Latest revision as of 11:19, 29 September 2025
Manuals and user guides
Current status
The current status of the microscope can be found here and on the booking website.
Biosafety and access
The SP8-Lia is located in an AP-1 room. See the Biosafety section on rules regarding working in this room.
Technical specifications
- Inverted DMi8 CS motorized microscope
- 2000 FOV scanner SP8
- Light source EL 6000
Filter Cubes
- DAPI LP (ex: 380-400, em: 425-infinite nm)
- GFP (ex: 450-490, em: 500-550)
- Y3 (ex: 533-558, em: 570-640)
- Y5 (ex: 590-650, em: 662-738)
Objectives
- Obj. HC PL APO 10x/0.40 CS2 (FWD = 2.56 mm)
- Obj. HC PL FLUOTAR L 20x/0.40 (7.5-6.2 mm)
- Obj. HC PL APO 20x/0.75 CS2 (0.66 mm)
- Obj. HC PL FLUOTAR L 40x/0.60 (3.3-1.9 mm)
- Obj. HC PL APO 40x/1.30 Oil CS2 (0.24 mm)
- Obj. HC PL APO 63x/1.40 OIL CS2 (0.14 mm)
Detectors
- 1 x PMT Detector
- 2 x HyD SP GaAsP Detector (sensitive)
- Brightfield detector for DMI
Lasers
- Laser 405 nm DMOD Compact
- Laser Blue 488 nm
- Laser Green 552 nm
- Laser Red 638 nm
Camera
- Leica DFC7000 GT monochrome camera
Software
- LAS X SP8 Control Software with Navigator Module