Nikon sora introduction protocol

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Introduction protocol

Type of microscope and its strengths - Spinning disc microscopy for fast confocal microscopy (optical sectioning) - Suitable for live-cell imaging experiments - Superresolution optional, to achieve (ideally) ca. 150 nm resolution in green. - Two-camera option for capturing two fluorescent colors at the same time (dynamic processes) - Fluorescence Recovery After Photobleaching (FRAP) to study molecular diffusion times

Stage-top incubation system for live-cell imaging (switch on before starting a live cell experiment) First manual operation and doing Brightfield (finding the sample) Epifluorescence mode by eye and by camera (quick checking fluorescent signals) Confocal Spinning Disc mode (in-depth camera settings) SoRa Superresolution to double resolving power ND Acquisition for automated multidimensional experiments, start with Z-stack because full SoRa pipeline needs this. Perfect Focus System for keeping/using exact focus levels in live view and during ND Acquisition experiments

FRAP (optional) Dual camera mode (optional)

Data saving (nd2 files) and transfering (SURF file sender) User account creation Booking system discussion

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