DM2500
The Leica-Microsystems DM2500 microscope present in room HG01.228 is an upright microscope equipped for imaging in transmission (bright field, phase-contrast [10-100x], dark-field [from 10-40x]) and fluorescence mode.
Manuals and user guides
Current status
The current status of the microscope can be found here and on the booking website.
Biosafety and access
The SP8-Lia is located in an AP-1 room. See the Biosafety section on rules regarding working in this room.
Technical specifications
The Leica-Microsystems DM2500 microscope present in room HG01.228 is an upright microscope equipped for imaging in transmission (bright field, phase-contrast [10-100x], dark-field [from 10-40x]) and fluorescence mode.
Objectives with magnification 2.5, 10, 20, 40 and 100x are available. Images can be recorded with a DCF7000T Leica sensitive color camera (up to 1920x1440 pixels) that can be operated for bright-field, but also fluorescence, phase-contrast and dark-field wide-field microscopy. Freely definable ROIs in combination with binning are possible.
Other functions beside simple acquisition of images involve image averaging, shading corrections,sharpening with active noise reduction, white and black balance, HDR acquisition, time lapse imaging and streaming mode.
The camera works together with the LASX software. The modules for image overlays, time-lapse and video streaming are present.
Fluorescent filters mounted on the system are:
- A (excitation: 340-380 nm, emission: >425)
- I3 (450-490, >515)
- N2.1 (515-560, >590)
- Y5 (590-650, 662-738)