Leica Thunder
Manuals and user guides
Biosafety and access
The SoRa is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.
Current status
The current status of the microscope can be found here and on the booking website.
Technical specifications
Microscope
Automated DMi8 (inverted) with hardware autofocus (AFC) and motorized polarizer.
Excitation Lines
Lumencor SPECTRA-X 7-line LED with the following available wavelengths:
- 395 nm
- 440 nm
- 470 nm
- 510 nm
- 550 nm
- 640 nm
Emission Selection
- DFT5 quad-cube for high speed fluorescence imaging (Blue, Green, Red, Far-red, or 100%) enabling specific capture of:
- 420-450 nm (eg. DAPI, Hoechst)
- 506-532 nm (eg. GFP, FITC)
- 581-607 nm (eg. RFP, TRITC, Cy3)
- 666-724 nm (eg. AlexaFluor 633, DRAQ5, Cy5)
- CFP filter cube to capture:
- 460-500 nm (e.g. CFP, TFP, Cerulean)
- YFP filter cube to capture:
- 520-550 nm (e.g. YFP, mVenus)
Detection
Leica DFC9000 GTC
- 4.2 MP sCMOS camera, 6.5 μm pixel size (19 mm sensor diagonal)
- 82% quantum efficiency, dark current of 0.14e-/p/sec
- ca. 90 frames per second acquisition speed
- extended dynamic range of 33000:1
Objectives
- 5x/0.12 Dry (working distance 14 mm)
- 10x/0.40 Dry (2.74 mm)
- 20x/0.80 Air (0.40 mm)
- 40x/1.10 Water (0.65 mm)
- 63x/1.20 Water (0.30 mm)
- 63x/1.30 Glycerol (0.28 mm)
Stage
High speed/precision quantum stage
Environmental Control
Incubator with temperature controller
Software Modules
- LAS X 3D for 3D rendering of acquired volumes
- “Navigator” for large tile scan
- “Thunder” for instant or volume computational clearing and improvement of resolution