Leica SP8 Liachroic intro
Introduction
- Welcome, outline of the introduction
- The General Instrumentation department conceptually and physically
- The room and the general layout
- The microscope conceptually (functions)
- The microscope physically (components, roughly)
Switch-on
- Quick inspection (everything looks ok visually?)
- Switch-on procedure of components (buttons numbered order)
- Log-in in Windows (user will get an account) and software starting
Stage and objective movement controls (XY stage, Z objectives)
Sample placement (sample types, coverslip towards the bottom, adjusting holder, put demo or user sample there, don't clamp it but let it rest (otherwise breaks when objective hits))
Brightfield mode (where does light come from, what do we expect to see)
Focusing (sample finding, and then moving around, warning: hitting lens to sample possible)
Epifluorescence mode (see visually light shining, see returning light, discuss different filters, user responsible for knowing their dyes and wavelengths, Searchlight spectra viewer)
Confocal scanning mode (gonna be a big part, mostly on computer now, switch in mood/user focus on screen not on microscope)