Leica Thunder

Biosafety and access

The SoRa is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.

Current status

The current status of the microscope can be found here and on the booking website.

Technical specifications

Microscope

Automated DMi8 (inverted) with hardware autofocus (AFC) and motorized polarizer.

Excitation Lines

Lumencor SPECTRA-X 7-line LED with the following available wavelengths:

• 395 nm
• 440 nm
• 470 nm
• 510 nm
• 550 nm
• 640 nm

Emission Selection

• DFT5 quad-cube for high speed fluorescence imaging (Blue, Green, Red, Far-red, or 100%) enabling specific capture of:
  • 420-450 nm (eg. DAPI, Hoechst)
  • 506-532 nm (eg. GFP, FITC)
  • 581-607 nm (eg. RFP, TRITC, Cy3)
  • 666-724 nm (eg. AlexaFluor 633, DRAQ5, Cy5)

• CFP filter cube to capture:
  • 460-500 nm (e.g. CFP, TFP, Cerulean)

• YFP filter cube to capture:
  • 520-550 nm (e.g. YFP, mVenus)

Detection

Leica DFC9000 GTC

• 4.2 MP sCMOS camera, 6.5 μm pixel size (19 mm sensor diagonal)
• 82% quantum efficiency, dark current of 0.14e-/p/sec
• ca. 90 frames per second acquisition speed
• extended dynamic range of 33000:1

Objectives

• 5x/0.12 Dry (working distance 14 mm)
• 10x/0.40 Dry (2.74 mm)
• 20x/0.80 Air (0.40 mm)
• 40x/1.10 Water (0.65 mm)
• 63x/1.20 Water (0.30 mm)
• 63x/1.30 Glycerol (0.28 mm)

Stage

High speed/precision quantum stage

Environmental Control

Incubator with temperature controller

Software Modules

• LAS X 3D for 3D rendering of acquired volumes
• “Navigator” for large tile scan
• “Thunder” for instant or volume computational clearing and improvement of resolution