Nikon sora functions: Difference between revisions
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- Brightfield (simple white light lamp, light coming from above the objective) | - Brightfield (simple white light lamp, light coming from above the objective) | ||
- Widefield epifluorsecence (specific excitation and emission colors, excitation light coming out of the objective) | - Widefield epifluorsecence (specific excitation and emission colors, excitation light coming out of the objective) | ||
Revision as of 12:49, 28 July 2025
What can the Nikon SoRa do?
Briefly: It can do live-cell, dual-color high-speed superresolution confocal imaging, in combination with FRAP (optional).
In more detail, it can do:
- Live-cell incubation (keeping temperature, humidity and CO2 at desired levels)
Observation by eye, using the binoculars:
- Brightfield (simple white light lamp, light coming from above the objective)
- Widefield epifluorsecence (specific excitation and emission colors, excitation light coming out of the objective)
Observation by camera, using the software:
- Brightfield - Widefield epifluorescence - Spinning disc confocal imaging (up to ca. 260 nm resolution) - Superresolution spinning disc confocal imaging (up to ca. 120 nm resolution) - FRAP (fluorescence recovery after photobleaching, 488 nm bleaching laser available)