Nikon sora introduction protocol: Difference between revisions
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Introduction protocol | Introduction protocol | ||
Type of microscope and its strengths | * Type of microscope and its strengths | ||
* Spinning disc microscopy for fast confocal microscopy (optical sectioning) | |||
* Suitable for live-cell imaging experiments | |||
* Superresolution optional, to achieve (ideally) ca. 150 nm resolution in green. | |||
* Two-camera option for capturing two fluorescent colors at the same time (dynamic processes) | |||
* Fluorescence Recovery After Photobleaching (FRAP) to study molecular diffusion times | |||
Stage-top incubation system for live-cell imaging (switch on before starting a live cell experiment) | Stage-top incubation system for live-cell imaging (switch on before starting a live cell experiment) | ||
First manual operation and doing Brightfield (finding the sample) | First manual operation and doing Brightfield (finding the sample) | ||
Epifluorescence mode by eye and by camera (quick checking fluorescent signals) | Epifluorescence mode by eye and by camera (quick checking fluorescent signals) | ||
Confocal Spinning Disc mode (in-depth camera settings) | Confocal Spinning Disc mode (in-depth camera settings) | ||
SoRa Superresolution to double resolving power | SoRa Superresolution to double resolving power | ||
ND Acquisition for automated multidimensional experiments, start with Z-stack because full SoRa pipeline needs this. | ND Acquisition for automated multidimensional experiments, start with Z-stack because full SoRa pipeline needs this. | ||
Perfect Focus System for keeping/using exact focus levels in live view and during ND Acquisition experiments | Perfect Focus System for keeping/using exact focus levels in live view and during ND Acquisition experiments | ||
FRAP (optional) | FRAP (optional) | ||
Dual camera mode (optional) | Dual camera mode (optional) | ||
Data saving (nd2 files) and transfering (SURF file sender) | Data saving (nd2 files) and transfering (SURF file sender) | ||
User account creation | User account creation | ||
Booking system discussion | Booking system discussion | ||
Revision as of 15:55, 26 May 2026
Introduction protocol
- Type of microscope and its strengths
- Spinning disc microscopy for fast confocal microscopy (optical sectioning)
- Suitable for live-cell imaging experiments
- Superresolution optional, to achieve (ideally) ca. 150 nm resolution in green.
- Two-camera option for capturing two fluorescent colors at the same time (dynamic processes)
- Fluorescence Recovery After Photobleaching (FRAP) to study molecular diffusion times
Stage-top incubation system for live-cell imaging (switch on before starting a live cell experiment)
First manual operation and doing Brightfield (finding the sample)
Epifluorescence mode by eye and by camera (quick checking fluorescent signals)
Confocal Spinning Disc mode (in-depth camera settings)
SoRa Superresolution to double resolving power
ND Acquisition for automated multidimensional experiments, start with Z-stack because full SoRa pipeline needs this.
Perfect Focus System for keeping/using exact focus levels in live view and during ND Acquisition experiments
FRAP (optional)
Dual camera mode (optional)
Data saving (nd2 files) and transfering (SURF file sender)
User account creation
Booking system discussion