Nikon sora functions: Difference between revisions

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What can the Nikon SoRa do?
== What can the Nikon SoRa do? ==
It can do live-cell, dual-color high-speed superresolution confocal imaging, in combination with FRAP


Briefly: It can do live-cell, dual-color high-speed superresolution confocal imaging, in combination with FRAP (optional).
=== In more detail: ===


In more detail, it can do:
* Live-cell incubation (keeping temperature, humidity and CO2 at desired levels)


- Live-cell incubation (keeping temperature, humidity and CO2 at desired levels)
=== Observation by eye, using the binoculars: ===


Observation by eye, using the binoculars:
* Brightfield (simple white light lamp, light coming from above the objective)
* Widefield epifluorsecence (specific excitation and emission colors, excitation light coming out of the objective)


- Brightfield (simple white light lamp, light coming from above the objective)
=== Observation by camera, using the software: ===


- Widefield epifluorsecence (specific excitation and emission colors, excitation light coming out of the objective)
* Brightfield
 
* Widefield epifluorescence
Observation by camera, using the software:
* Spinning disc confocal imaging (up to ca. 260 nm resolution)
 
* Superresolution spinning disc confocal imaging (up to ca. 120 nm resolution)
- Brightfield
* FRAP (fluorescence recovery after photobleaching, 488 nm bleaching laser available)
- Widefield epifluorescence
- Spinning disc confocal imaging (up to ca. 260 nm resolution)
- Superresolution spinning disc confocal imaging (up to ca. 120 nm resolution)
- FRAP (fluorescence recovery after photobleaching, 488 nm bleaching laser available)

Latest revision as of 12:52, 28 July 2025

What can the Nikon SoRa do?

It can do live-cell, dual-color high-speed superresolution confocal imaging, in combination with FRAP

In more detail:

  • Live-cell incubation (keeping temperature, humidity and CO2 at desired levels)

Observation by eye, using the binoculars:

  • Brightfield (simple white light lamp, light coming from above the objective)
  • Widefield epifluorsecence (specific excitation and emission colors, excitation light coming out of the objective)

Observation by camera, using the software:

  • Brightfield
  • Widefield epifluorescence
  • Spinning disc confocal imaging (up to ca. 260 nm resolution)
  • Superresolution spinning disc confocal imaging (up to ca. 120 nm resolution)
  • FRAP (fluorescence recovery after photobleaching, 488 nm bleaching laser available)