Nikon sora switch on: Difference between revisions

Latest revision as of 07:52, 28 July 2025

1) Controller: powers the microscope stand

2) Temp. incubation - even if you don't need temp incubation; switch off the heater using its button on this box

5) Laser box - lasers are required to image

7) Cam 1 - at least 1 camera is required to image

9) SoRa unit - this is required for confocal imaging

10) PC - required for using the camera(s), multidimensional acquisitions, any confocal experiments

3) Humidity + CO2 - only when live-cell imaging samples that require this controlled - make sure to add water to the incubator

4) FRAP scanner - only when intending to do FRAP (fluorescence recovery after photobleaching) experiments

6) FRAP interlock - only when intending to do FRAP experiments

8) Cam 2 - only when intending to do simultaneous 2-color imaging experiments

@@ Line 1: / Line 1: @@
 [[File:Sora_schematic_switch_on.png|1000x1000px]]
+=== Required elements: ===
+) '''Controller''': powers the microscope stand
+) '''Temp. incubation''' - even if you don't need temp incubation; switch off the heater using its button on this box
+) '''Laser box''' - lasers are required to image
+) '''Cam 1''' - at least 1 camera is required to image
+) '''SoRa unit''' - this is required for confocal imaging
+) '''PC''' - required for using the camera(s), multidimensional acquisitions, any confocal experiments
+=== Optional elements: ===
+) '''Humidity + CO2''' - only when live-cell imaging samples that require this controlled - make sure to add water to the incubator
+) '''FRAP scanner''' - only when intending to do [[wikipedia:Fluorescence_recovery_after_photobleaching|FRAP]] (fluorescence recovery after photobleaching) experiments
+) '''FRAP interlock -''' only when intending to do FRAP experiments
+) '''Cam 2''' - only when intending to do simultaneous 2-color imaging experiments