Nikon sora superresolution switch: Difference between revisions
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# | How to switch from normal to superresolution: | ||
# | # Use the '''100x / 1.49 oil''' objective (is the highest numerical aperture lens available) | ||
# | # Switch to the '''SoRa spinning disc''' under "pinhole" | ||
# | # Switch to the '''2.8x SoRa magnifier''' | ||
# | # Set the camera to "'''binning 1x1'''" (no binning) | ||
# | # '''Obtain a Z-stack''': Make the stack slightly "too big" - having some extra blurry layers above/below the structure helps with deconvolution afterwards. | ||
# Perform "'''3D Deconvolution'''" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed) | |||
# '''Evaluate''' result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution) | |||
How to switch back to normal imaging again: | |||
# Switch back to '''50 um''' pinhole (not SoRa) | |||
# Switch back to the '''1x''' '''SoRa magnifier''' (not 2.8x or 4.0x) | |||
# Taking z-stacks/using no camera binning is not essential anymore here | |||
Latest revision as of 13:56, 4 July 2025
How to switch from normal to superresolution:
- Use the 100x / 1.49 oil objective (is the highest numerical aperture lens available)
- Switch to the SoRa spinning disc under "pinhole"
- Switch to the 2.8x SoRa magnifier
- Set the camera to "binning 1x1" (no binning)
- Obtain a Z-stack: Make the stack slightly "too big" - having some extra blurry layers above/below the structure helps with deconvolution afterwards.
- Perform "3D Deconvolution" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed)
- Evaluate result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)
How to switch back to normal imaging again:
- Switch back to 50 um pinhole (not SoRa)
- Switch back to the 1x SoRa magnifier (not 2.8x or 4.0x)
- Taking z-stacks/using no camera binning is not essential anymore here