Nikon sora: Difference between revisions

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[[Nikon sora functions|What can it do?]]
[[Nikon sora functions|What can it do?]]


[[Nikon sora switch on|How to '''switch on''']]
[[Nikon sora introduction protocol|Introduction protocol]]


[[Nikon sora superresolution switch|How to '''switch to superresolution''']]
[[Nikon sora switch on|How to switch on]]
 
[[Nikon sora superresolution switch|How to switch to superresolution]]


[[Nikon sora lightpath|Dual camera imaging wavelengths]]
[[Nikon sora lightpath|Dual camera imaging wavelengths]]

Latest revision as of 15:53, 26 May 2026

Manuals and user guides

What can it do?

Introduction protocol

How to switch on

How to switch to superresolution

Dual camera imaging wavelengths

Tips & Tricks

Current status

The current status of the microscope can be found here and on the booking website.

Biosafety and access

The SoRa is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.

Technical specifications

Available hardware

  • 20x air obj.
  • 40x/1.30 oil (working distance 200 um)
  • 100x/1.49 oil (w.d. 160 um)

Superresolution components

  • SoRa spinning disc
  • Additional SoRa magnifiers 2.8x / 4.0x
  • 3D deconvolution software (in-line and parallel)

Imaging lasers

  • 405 nm
  • 445 nm
  • 488 nm
  • 561 nm
  • 638 nm

Emission filters

  • UV
  • Green
  • Yellow
  • Red
  • Far-red

Dual color simultaneous imaging

  • blue/yellow (e.g. CFP / YFP)
  • green/red (e.g. GFP / RFP)

Software

  • NIS-elements
  • Nikon batch-deconvolution

Other

  • FURA (calcium sensor) imaging filters and light source
  • "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)