Nikon sora: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| (One intermediate revision by one other user not shown) | |||
| Line 7: | Line 7: | ||
[[Nikon sora lightpath|Dual camera imaging wavelengths]] | [[Nikon sora lightpath|Dual camera imaging wavelengths]] | ||
[[Nikon sora tips tricks|Tips & Tricks]] | |||
== Current status == | == Current status == | ||
| Line 16: | Line 18: | ||
== Technical specifications == | == Technical specifications == | ||
=== Available hardware | === Available hardware === | ||
* 20x air obj. | * 20x air obj. | ||
| Line 22: | Line 24: | ||
* 100x/1.49 oil (w.d. 160 um) | * 100x/1.49 oil (w.d. 160 um) | ||
=== Superresolution components | === Superresolution components === | ||
* SoRa spinning disc | * SoRa spinning disc | ||
| Line 28: | Line 30: | ||
* 3D deconvolution software (in-line and parallel) | * 3D deconvolution software (in-line and parallel) | ||
=== Imaging lasers | === Imaging lasers === | ||
* 405 nm | * 405 nm | ||
| Line 36: | Line 38: | ||
* 638 nm | * 638 nm | ||
=== Emission filters | === Emission filters === | ||
* UV | * UV | ||
| Line 44: | Line 46: | ||
* Far-red | * Far-red | ||
=== Dual color simultaneous imaging | === Dual color simultaneous imaging === | ||
* blue/yellow (e.g. CFP / YFP) | * blue/yellow (e.g. CFP / YFP) | ||
* green/red (e.g. GFP / RFP) | * green/red (e.g. GFP / RFP) | ||
=== Software | === Software === | ||
* NIS-elements | * NIS-elements | ||
* Nikon batch-deconvolution | * Nikon batch-deconvolution | ||
=== Other | === Other === | ||
* FURA (calcium sensor) imaging filters and light source | * FURA (calcium sensor) imaging filters and light source | ||
* "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control) | * "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control) | ||
Latest revision as of 07:51, 16 October 2025
Manuals and user guides
How to switch to superresolution
Dual camera imaging wavelengths
Current status
The current status of the microscope can be found here and on the booking website.
Biosafety and access
The SoRa is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.
Technical specifications
Available hardware
- 20x air obj.
- 40x/1.30 oil (working distance 200 um)
- 100x/1.49 oil (w.d. 160 um)
Superresolution components
- SoRa spinning disc
- Additional SoRa magnifiers 2.8x / 4.0x
- 3D deconvolution software (in-line and parallel)
Imaging lasers
- 405 nm
- 445 nm
- 488 nm
- 561 nm
- 638 nm
Emission filters
- UV
- Green
- Yellow
- Red
- Far-red
Dual color simultaneous imaging
- blue/yellow (e.g. CFP / YFP)
- green/red (e.g. GFP / RFP)
Software
- NIS-elements
- Nikon batch-deconvolution
Other
- FURA (calcium sensor) imaging filters and light source
- "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)