Nikon sora superresolution switch: Difference between revisions

Revision as of 13:52, 4 July 2025

Use the 100x / 1.49 oil objective
Enable the 2.8x SoRa magnifier
Set camera to "binning 1x1" (no binning)
Obtain a Z-stack: Make the stack slightly "too big" - having some extra blurry layers above/below the structure helps with deconvolution afterwards.
Perform "3D Deconvolution" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed)
Evaluate result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)

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-# 1) Use the 100x / 1.49 oil lens
+# Use the '''100x / 1.49 oil''' objective
-# 2) Enable the 2.8x SoRa magnifier
+# Enable the '''2.8x SoRa magnifier'''
-# 3) Set camera to "binning 1x1" (no binning)
+# Set camera to "'''binning 1x1'''" (no binning)
-# 4) Obtain a Z-stack: Include lots of blur above / below the interesting focus layers
+# '''Obtain a Z-stack''': Make the stack slightly "too big" - having some extra blurry layers above/below the structure helps with deconvolution afterwards.
-# 5) Perform "3D Deconvolution" (software option) with the standard settings.
+# Perform "'''3D Deconvolution'''" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed)
-# 6) Evaluate result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)
+# '''Evaluate''' result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)