Nikon sora: Difference between revisions

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== Manuals and user guides ==
[[Nikon sora functions|What can it do?]]
[[Nikon sora functions|What can it do?]]


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[[Nikon sora lightpath|Dual camera imaging wavelengths]]
[[Nikon sora lightpath|Dual camera imaging wavelengths]]


=== Biosafety and access ===
[[Nikon sora tips tricks|Tips & Tricks]]


== Current status ==
The current status of the microscope can be found [[Current microscope status#Light microscopes|here]] and on the [https://bookings.science.ru.nl/category/general-instrumentation-gi/gi-light-microscopy-image-analysis booking website].


== Biosafety and access ==
The SoRa is located in an ML-1 room. See the [[General Instrumentation Department#Biosafety|Biosafety]] section on rules regarding working in this room, and how to get access.
The SoRa is located in an ML-1 room. See the [[General Instrumentation Department#Biosafety|Biosafety]] section on rules regarding working in this room, and how to get access.
== Technical specifications ==
=== Available hardware ===
* 20x air obj.
* 40x/1.30 oil (working distance 200 um)
* 100x/1.49 oil (w.d. 160 um)
=== Superresolution components ===
* SoRa spinning disc
* Additional SoRa magnifiers 2.8x / 4.0x
* 3D deconvolution software (in-line and parallel)
=== Imaging lasers ===
* 405 nm
* 445 nm
* 488 nm
* 561 nm
* 638 nm
=== Emission filters ===
* UV
* Green
* Yellow
* Red
* Far-red
=== Dual color simultaneous imaging ===
* blue/yellow (e.g. CFP / YFP)
* green/red (e.g. GFP / RFP)
=== Software ===
* NIS-elements
* Nikon batch-deconvolution
=== Other ===
* FURA (calcium sensor) imaging filters and light source
* "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)

Latest revision as of 07:51, 16 October 2025

Manuals and user guides

What can it do?

How to switch on

How to switch to superresolution

Dual camera imaging wavelengths

Tips & Tricks

Current status

The current status of the microscope can be found here and on the booking website.

Biosafety and access

The SoRa is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.

Technical specifications

Available hardware

  • 20x air obj.
  • 40x/1.30 oil (working distance 200 um)
  • 100x/1.49 oil (w.d. 160 um)

Superresolution components

  • SoRa spinning disc
  • Additional SoRa magnifiers 2.8x / 4.0x
  • 3D deconvolution software (in-line and parallel)

Imaging lasers

  • 405 nm
  • 445 nm
  • 488 nm
  • 561 nm
  • 638 nm

Emission filters

  • UV
  • Green
  • Yellow
  • Red
  • Far-red

Dual color simultaneous imaging

  • blue/yellow (e.g. CFP / YFP)
  • green/red (e.g. GFP / RFP)

Software

  • NIS-elements
  • Nikon batch-deconvolution

Other

  • FURA (calcium sensor) imaging filters and light source
  • "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)