Leica SP8X

The SP8x confocal laser scanning microscope (Leica-microsystems) is mounted on a fully motorized DMi8 microscope on which x, y, z, t; lambda (wavelength), and sequential scans can be acquired at multiple positions and in z-stack. There are functions for stitching, and digital “best focus” determination also in z-stacks. Deconvolution can be done on the attached CUDA PC equipped with the LAS X module "Lightning". Live-cell imaging can be performed using a closed lid that controls CO2 and relative humidity levels, and using the big incubator box that controls temperature and airflow.

Current status

The current status of the microscope can be found here and on the booking website.

Biosafety and access

The SP8-Lia is located in an AP-1 room. See the Biosafety section on rules regarding working in this room.

Technical specifications

Objectives

• 10x / 0.40 Air (FWD = 2.2 mm)
• 20x / 0.40 Air (7.5 mm long distance)
• 40x / 0.60 Air (3.3 mm long distance)
• 40x / 1.10 Water (0.62 mm) - motorCorrection
• 63x / 1.40 Oil (0.14 mm)
• 100x / 1.40 Oil (0.13 mm)

Lasers

• 405 nm diode laser + pulsed White Light Laser (WLL), up to 8 tunable excitation lines from 470-670nm can be applied simultaneously

Detectors and camera

• 2 GaAsP PMTs
• 2 HyDs (high sensitivity, linear photon counting and time-gating)
• 1 normal transmitted-light PMT

In addition, the DMi8 microscope can be utilized for wide-field bright field and fluorescence observation. Images can be acquired with a monochrome DFC365FX camera.

Filter cubes

• DAPI (LP425)
• FITC (LP515)
• Rhod (LP590)
• Y5 (BP 662-738)