Nikon sora: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| (9 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
== Manuals and user guides == | |||
[[Nikon sora functions|What can it do?]] | [[Nikon sora functions|What can it do?]] | ||
| Line 4: | Line 5: | ||
[[Nikon sora superresolution switch|How to '''switch to superresolution''']] | [[Nikon sora superresolution switch|How to '''switch to superresolution''']] | ||
[[Nikon sora lightpath|Dual camera imaging wavelengths]] | |||
== Current status == | |||
The current status of the microscope can be found [[Current microscope status#Light microscopes|here]] and on the [https://bookings.science.ru.nl/category/general-instrumentation-gi/gi-light-microscopy-image-analysis booking website]. | |||
== Biosafety and access == | |||
The SoRa is located in an ML-1 room. See the [[General Instrumentation Department#Biosafety|Biosafety]] section on rules regarding working in this room, and how to get access. | |||
== Technical specifications == | |||
=== Available hardware === | |||
* 20x air obj. | |||
* 40x/1.30 oil (working distance 200 um) | |||
* 100x/1.49 oil (w.d. 160 um) | |||
=== Superresolution components === | |||
* SoRa spinning disc | |||
* Additional SoRa magnifiers 2.8x / 4.0x | |||
* 3D deconvolution software (in-line and parallel) | |||
=== Imaging lasers === | |||
* 405 nm | |||
* 445 nm | |||
* 488 nm | |||
* 561 nm | |||
* 638 nm | |||
=== Emission filters === | |||
* UV | |||
* Green | |||
* Yellow | |||
* Red | |||
* Far-red | |||
=== Dual color simultaneous imaging === | |||
* blue/yellow (e.g. CFP / YFP) | |||
* green/red (e.g. GFP / RFP) | |||
=== Software === | |||
* NIS-elements | |||
* Nikon batch-deconvolution | |||
=== Other === | |||
* FURA (calcium sensor) imaging filters and light source | |||
* "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control) | |||
Latest revision as of 11:12, 29 September 2025
Manuals and user guides
How to switch to superresolution
Dual camera imaging wavelengths
Current status
The current status of the microscope can be found here and on the booking website.
Biosafety and access
The SoRa is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.
Technical specifications
Available hardware
- 20x air obj.
- 40x/1.30 oil (working distance 200 um)
- 100x/1.49 oil (w.d. 160 um)
Superresolution components
- SoRa spinning disc
- Additional SoRa magnifiers 2.8x / 4.0x
- 3D deconvolution software (in-line and parallel)
Imaging lasers
- 405 nm
- 445 nm
- 488 nm
- 561 nm
- 638 nm
Emission filters
- UV
- Green
- Yellow
- Red
- Far-red
Dual color simultaneous imaging
- blue/yellow (e.g. CFP / YFP)
- green/red (e.g. GFP / RFP)
Software
- NIS-elements
- Nikon batch-deconvolution
Other
- FURA (calcium sensor) imaging filters and light source
- "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)