Nikon sora: Difference between revisions
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== Technical specifications == | == Technical specifications == | ||
=== Available hardware | === Available hardware === | ||
* 20x air obj. | * 20x air obj. | ||
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* 100x/1.49 oil (w.d. 160 um) | * 100x/1.49 oil (w.d. 160 um) | ||
=== Superresolution components | === Superresolution components === | ||
* SoRa spinning disc | * SoRa spinning disc | ||
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* 3D deconvolution software (in-line and parallel) | * 3D deconvolution software (in-line and parallel) | ||
=== Imaging lasers | === Imaging lasers === | ||
* 405 nm | * 405 nm | ||
| Line 36: | Line 36: | ||
* 638 nm | * 638 nm | ||
=== Emission filters | === Emission filters === | ||
* UV | * UV | ||
| Line 44: | Line 44: | ||
* Far-red | * Far-red | ||
=== Dual color simultaneous imaging | === Dual color simultaneous imaging === | ||
* blue/yellow (e.g. CFP / YFP) | * blue/yellow (e.g. CFP / YFP) | ||
* green/red (e.g. GFP / RFP) | * green/red (e.g. GFP / RFP) | ||
=== Software | === Software === | ||
* NIS-elements | * NIS-elements | ||
* Nikon batch-deconvolution | * Nikon batch-deconvolution | ||
=== Other | === Other === | ||
* FURA (calcium sensor) imaging filters and light source | * FURA (calcium sensor) imaging filters and light source | ||
* "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control) | * "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control) | ||
Latest revision as of 11:12, 29 September 2025
Manuals and user guides
How to switch to superresolution
Dual camera imaging wavelengths
Current status
The current status of the microscope can be found here and on the booking website.
Biosafety and access
The SoRa is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.
Technical specifications
Available hardware
- 20x air obj.
- 40x/1.30 oil (working distance 200 um)
- 100x/1.49 oil (w.d. 160 um)
Superresolution components
- SoRa spinning disc
- Additional SoRa magnifiers 2.8x / 4.0x
- 3D deconvolution software (in-line and parallel)
Imaging lasers
- 405 nm
- 445 nm
- 488 nm
- 561 nm
- 638 nm
Emission filters
- UV
- Green
- Yellow
- Red
- Far-red
Dual color simultaneous imaging
- blue/yellow (e.g. CFP / YFP)
- green/red (e.g. GFP / RFP)
Software
- NIS-elements
- Nikon batch-deconvolution
Other
- FURA (calcium sensor) imaging filters and light source
- "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)