Nikon sora: Difference between revisions

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== Manuals and user guides ==
[[Nikon sora functions|What can it do?]]
[[Nikon sora functions|What can it do?]]


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== Technical specifications ==
== Technical specifications ==


=== Available hardware: ===
=== Available hardware ===


* 20x air obj.
* 20x air obj.
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* 100x/1.49 oil (w.d. 160 um)
* 100x/1.49 oil (w.d. 160 um)


=== Superresolution components: ===
=== Superresolution components ===


* SoRa spinning disc
* SoRa spinning disc
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* 3D deconvolution software (in-line and parallel)
* 3D deconvolution software (in-line and parallel)


=== Imaging lasers: ===
=== Imaging lasers ===


* 405 nm
* 405 nm
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* 638 nm
* 638 nm


=== Emission filters: ===
=== Emission filters ===


* UV
* UV
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* Far-red
* Far-red


=== Dual color simultaneous imaging: ===
=== Dual color simultaneous imaging ===


* blue/yellow (e.g. CFP / YFP)
* blue/yellow (e.g. CFP / YFP)
* green/red (e.g. GFP / RFP)
* green/red (e.g. GFP / RFP)


=== Software: ===
=== Software ===


* NIS-elements
* NIS-elements
* Nikon batch-deconvolution
* Nikon batch-deconvolution


=== Other: ===
=== Other ===


* FURA (calcium sensor) imaging filters and light source
* FURA (calcium sensor) imaging filters and light source
* "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)
* "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)

Latest revision as of 11:12, 29 September 2025

Manuals and user guides

What can it do?

How to switch on

How to switch to superresolution

Dual camera imaging wavelengths

Current status

The current status of the microscope can be found here and on the booking website.

Biosafety and access

The SoRa is located in an ML-1 room. See the Biosafety section on rules regarding working in this room, and how to get access.

Technical specifications

Available hardware

  • 20x air obj.
  • 40x/1.30 oil (working distance 200 um)
  • 100x/1.49 oil (w.d. 160 um)

Superresolution components

  • SoRa spinning disc
  • Additional SoRa magnifiers 2.8x / 4.0x
  • 3D deconvolution software (in-line and parallel)

Imaging lasers

  • 405 nm
  • 445 nm
  • 488 nm
  • 561 nm
  • 638 nm

Emission filters

  • UV
  • Green
  • Yellow
  • Red
  • Far-red

Dual color simultaneous imaging

  • blue/yellow (e.g. CFP / YFP)
  • green/red (e.g. GFP / RFP)

Software

  • NIS-elements
  • Nikon batch-deconvolution

Other

  • FURA (calcium sensor) imaging filters and light source
  • "Tokai Hit" Stage-top live-cell incubation system (CO2, temperature and humidity control)