https://wiki.gi.science.ru.nl/index.php?action=history&feed=atom&title=PhaseView_light_sheetPhaseView light sheet - Revision history2026-05-09T07:08:28ZRevision history for this page on the wikiMediaWiki 1.43.1https://wiki.gi.science.ru.nl/index.php?title=PhaseView_light_sheet&diff=209&oldid=prevHetty: Started page2025-09-29T11:59:30Z<p>Started page</p>
<p><b>New page</b></p><div>PhaseView/Evident light sheet microscope with customized sample holders (in SL-1 room). <br />
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== Manuals and user guides ==<br />
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== Current status ==<br />
The current status of the microscope can be found [[Current microscope status#Light microscopes|here]] and on the [https://bookings.science.ru.nl/category/general-instrumentation-gi/gi-light-microscopy-image-analysis booking website].<br />
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== Biosafety and access ==<br />
The Timelapse microscope is located in an SL-1 room. GMO's are not allowed in this room.<br />
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== Technical specifications ==<br />
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=== Microscope ===<br />
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* Manual upright Olympus BX43F microscope<br />
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=== Excitation ===<br />
Dual light sheet illumination through two 10x/0.25NA long distance Olympus objectives using a 4-laser combiner of the following laser lines:<br />
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* 405nm/50mW<br />
* 488nm/50mW<br />
* 561nm/50mW<br />
* 638nm/100mW<br />
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=== Emission Selection ===<br />
The system has the following filters mounted on a motorized filter wheel:<br />
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* ET440/40 nm<br />
* ET525/50<br />
* ET600/50<br />
* ET690/50<br />
* Notch filter 405/488/561/638<br />
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=== Detection ===<br />
ORCA-FLASH4.0V3<br />
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* Scientific CMOS Sensor 2048 (H)×2048 (V), 6.5 μm×6.5 μm<br />
* QE 82 % (@560 nm)<br />
* Format 13.312 mm×13.312 mm<br />
* Camera Link interface (100fps)<br />
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=== Objectives ===<br />
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* 10x/0.3 Water dipping long distance<br />
* 20x/0.5 Water dipping long distance<br />
* 40x/0.8 Water dipping long distance<br />
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=== Chamber and Sample holders ===<br />
Chamber dimensions: width 21mm, length 70m height 25mm. Sample size up to 10 x 10 x 10 mm, cuvette filled with watery refractive index media (volume < 15ml. Agarose strips containing sampes can be mounted in water and observed straight away.<br />
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=== Motorized Rotation Module ===<br />
Motorized rotation module with 1024 steps resolution, software controlled.<br />
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=== XY Tiling ===<br />
XY motorized stage with 15mm range and 0.1um precision. Software controlled<br />
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=== Software ===<br />
QtSPIM software for image acquisition XY, Z, channels, t (time), θ (rotation) and tiling</div>Hetty