Nikon sora superresolution switch - Revision history

Jelle at 13:56, 4 July 2025

2025-07-04T13:56:19Z

← Older revision		Revision as of 13:56, 4 July 2025
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	How to switch from normal ~~mode~~		How to switch from normal to superresolution:
	# Use the '''100x / 1.49 oil''' objective (is the highest numerical aperture lens available)		# Use the '''100x / 1.49 oil''' objective (is the highest numerical aperture lens available)
	# Switch to the '''SoRa spinning disc''' under "pinhole"		# Switch to the '''SoRa spinning disc''' under "pinhole"
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	# Perform "'''3D Deconvolution'''" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed)		# Perform "'''3D Deconvolution'''" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed)
	# '''Evaluate''' result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)		# '''Evaluate''' result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)
			How to switch back to normal imaging again:

			# Switch back to '''50 um''' pinhole (not SoRa)
			# Switch back to the '''1x''' '''SoRa magnifier''' (not 2.8x or 4.0x)
			# Taking z-stacks/using no camera binning is not essential anymore here

Jelle at 13:53, 4 July 2025

2025-07-04T13:53:46Z

← Older revision		Revision as of 13:53, 4 July 2025
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	# Use the '''100x / 1.49 oil''' objective		How to switch from normal mode
	# ~~Enable~~ the '''2.8x SoRa magnifier'''		# Use the '''100x / 1.49 oil''' objective (is the highest numerical aperture lens available)
	# Set camera to "'''binning 1x1'''" (no binning)		# Switch to the '''SoRa spinning disc''' under "pinhole"
			# Switch to the '''2.8x SoRa magnifier'''
			# Set the camera to "'''binning 1x1'''" (no binning)
	# '''Obtain a Z-stack''': Make the stack slightly "too big" - having some extra blurry layers above/below the structure helps with deconvolution afterwards.		# '''Obtain a Z-stack''': Make the stack slightly "too big" - having some extra blurry layers above/below the structure helps with deconvolution afterwards.
	# Perform "'''3D Deconvolution'''" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed)		# Perform "'''3D Deconvolution'''" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed)
	# '''Evaluate''' result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)		# '''Evaluate''' result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)

Jelle at 13:52, 4 July 2025

2025-07-04T13:52:22Z

← Older revision		Revision as of 13:52, 4 July 2025
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	# 1) Use the 100x / 1.49 oil ~~lens~~		# Use the '''100x / 1.49 oil''' objective
	# 2) Enable the 2.8x SoRa magnifier		# Enable the '''2.8x SoRa magnifier'''
	# 3) Set camera to "binning 1x1" (no binning)		# Set camera to "'''binning 1x1'''" (no binning)
	# 4) Obtain a Z-stack: ~~Include lots of blur~~ above / below the ~~interesting focus layers~~		# '''Obtain a Z-stack''': Make the stack slightly "too big" - having some extra blurry layers above/below the structure helps with deconvolution afterwards.
	# 5) Perform "3D Deconvolution" (software ~~option~~) with the standard settings.		# Perform "'''3D Deconvolution'''" (software command) with the standard suggestged settings (mostly change "noisyness" of the picture when needed)
	# 6) Evaluate result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)		# '''Evaluate''' result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)

Jelle: first add

2025-07-04T13:49:48Z

first add

New page

# 1) Use the 100x / 1.49 oil lens
# 2) Enable the 2.8x SoRa magnifier
# 3) Set camera to "binning 1x1" (no binning)
# 4) Obtain a Z-stack: Include lots of blur above / below the interesting focus layers
# 5) Perform "3D Deconvolution" (software option) with the standard settings.
# 6) Evaluate result and adjust parameters where necessary (e.g. adjust Z-step size, adjust image "noisyness" setting upon deconvolution)