https://wiki.gi.science.ru.nl/index.php?action=history&feed=atom&title=DM2500DM2500 - Revision history2026-05-02T05:26:50ZRevision history for this page on the wikiMediaWiki 1.43.1https://wiki.gi.science.ru.nl/index.php?title=DM2500&diff=212&oldid=prevHetty: Started page2025-09-29T12:03:18Z<p>Started page</p>
<p><b>New page</b></p><div>The Leica-Microsystems DM2500 microscope present in room HG01.228 is an upright microscope equipped for imaging in transmission (bright field, phase-contrast [10-100x], dark-field [from 10-40x]) and fluorescence mode. <br />
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== Manuals and user guides ==<br />
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== Current status ==<br />
The current status of the microscope can be found [[Current microscope status#Light microscopes|here]] and on the [https://bookings.science.ru.nl/category/general-instrumentation-gi/gi-light-microscopy-image-analysis booking website].<br />
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== Biosafety and access ==<br />
The SP8-Lia is located in an AP-1 room. See the [[General Instrumentation Department#Biosafety|Biosafety]] section on rules regarding working in this room.<br />
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== Technical specifications ==<br />
The Leica-Microsystems DM2500 microscope present in room HG01.228 is an upright microscope equipped for imaging in transmission (bright field, phase-contrast [10-100x], dark-field [from 10-40x]) and fluorescence mode. <br />
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Objectives with magnification 2.5, 10, 20, 40 and 100x are available. Images can be recorded with a DCF7000T Leica sensitive color camera (up to 1920x1440 pixels) that can be operated for bright-field, but also fluorescence, phase-contrast and dark-field wide-field microscopy. Freely definable ROIs in combination with binning are possible.<br />
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Other functions beside simple acquisition of images involve image averaging, shading corrections,sharpening with active noise reduction, white and black balance, HDR acquisition, time lapse imaging and streaming mode.<br />
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The camera works together with the LASX software. The modules for image overlays, time-lapse and video streaming are present. <br />
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Fluorescent filters mounted on the system are:<br />
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* A (excitation: 340-380 nm, emission: >425)<br />
* I3 (450-490, >515)<br />
* N2.1 (515-560, >590)<br />
* Y5 (590-650, 662-738)</div>Hetty